| assay
name |
description |
cost of
reagents per assay |
cost of
labor per assay |
total cost
per assay |
total cost
per assay for DERC member |
| |
|
|
|
|
|
| PCR |
|
|
|
|
|
| PCR primer set design and synthesis |
identify
gene in database, select primer sequences and synthesis |
10 |
11.5 |
21.5 |
13.5 |
| PCR without gel analysis (for >10 samples per primer set) |
PCR reaction |
0.25 |
0.75 |
1 |
0.5 |
| with agarose gel
analysis |
analysis by agarose gel |
0.45 |
2.55 |
3 |
1.2 |
| Quantitative PCR analysis (first sample with set up fee) |
use ABI real-time reagent, in duplicates, with standard curve |
24.8 |
11.5 |
36.3 |
28.3 |
| addition samples in the same sample set |
|
4.8 |
1.1 |
5.9 |
5.1 |
| |
|
|
|
|
|
| DNA Prep and Analysis |
|
|
|
|
|
| ear or tail DNA
extraction |
tissue provided by users, small amount of DNA ready for PCR
analysis |
0.15 |
0.75 |
0.9 |
0.4 |
| tail DNA extraction and PCR genotyping (for >10 animals) |
DNA extaction, PCR and agarose gel analysis |
0.6 |
3.3 |
3.9 |
1.6 |
| genomic DNA isolation |
high quality genomic DNA (>100 ug) |
2.5 |
11.5 |
14 |
6.0 |
| additional sample (in the
same sample set) |
|
2.5 |
2.5 |
5 |
3.3 |
| DNA analysis by Southern blotting (up to 18 sample-digestion) |
DNA
provided by users, resitriction, agarose gel, transfer, synthesize probe and
hybridization and detection |
54 |
195 |
249 |
112.5 |
| |
|
|
|
|
|
| RNA Prep and Analysis |
|
|
|
|
|
| RNA extraction |
use Qiagen mini RNAeasy kit |
2 |
2.3 |
4.3 |
2.7 |
| Reverse transcription |
random primer or primer provided by user , Supercript RTase |
15 |
1.6 |
16.6 |
15.5 |
| semi quantitative
PCR analysis of RT |
use ABI real-time reagent, in duplicates, 18srRNA or beta-actin as control |
9.6 |
2.2 |
11.8 |
10.3 |
| RNA
exp by Northern blotting (up to 18 samples) |
RNA
provided by users, agarose gel, transfer, synthesize probe, hybridization and
detection |
40 |
184 |
224 |
95.2 |
| Human Expression Array analysis (not including costs of Chips
and data analysis) |
RNA
(provided by users) reverse transcribed, labled, fragmented, hybridized and
scanned |
145 |
20.1 |
165.1 |
151.0 |
| Mouse Expression Array analysis (not including costs of Chips
and data analysis) |
RNA
(provided by users) reverse transcribed, labled, fragmented, hybridized and
scanned |
145 |
20.1 |
165.1 |
151.0 |
| |
|
|
|
|
|
| Mutation Detection |
|
|
|
|
|
| PCR/restriction analysis (for >10 samples) |
PCR, restrict digestion, agarose gel |
1.95 |
3 |
4.95 |
2.9 |
| direct sequencing |
DNA sequencing reaction |
5 |
|
5 |
5.0 |
| |
DNA sequencing reaction,
with analysis of raw data |
5 |
5.75 |
10.75 |
6.7 |
| pyrosequencing |
with analysis of raw data, final data in spread sheet |
2.34 |
6.5 |
8.84 |
4.3 |
| FPTDI |
with analysis of raw data, final data in spread sheet |
0.79 |
5.75 |
6.54 |
2.5 |
| DHPLC |
with analysis of raw data, final data in spread sheet |
2 |
5.75 |
7.75 |
3.7 |
| human 500K SNP assay (not including costs of Chips and data
analysis) |
DNA (provided by users) amplified, labled,
hybridized and scanned |
80 |
20.1 |
100.1 |
86.0 |
| mouse 5K SNP assay (not including costs of Chips and data
analysis) |
DNA
(provided by users) amplified, labled, hybridized and scanned |
80 |
20.1 |
100.1 |
86.0 |
|
|
|
|
|
|
